importantly, the obtained data may be analyzed obiectively, We use the ACEA system to follow growth of tumors cells in response to kinase inhibitors, to check resistance to drugs and inhibitors. Founded in 2002, ACEA Biosciences is a pioneer in the development and commercialization of high performance, cutting-edge cell analysis platforms for life science research. I would recommend it for cellular biology assays. Nov 14, 2014 xCELLigence SP - Agilent technologies Basic tool with a huge impact for any cell based assay The RTCA SP Station is located inside a tissue culture incubator and is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement. Unlike end-point assays this continuous monitoring of cell growth/death gives a better understanding of the biological process. The xCELLigence RTCA S16 instrument uses non invasive electrical impedance monitoring to quantify cell proliferation, morphology change, and attachment quality in a label-free, real-time manner.. Very user friendly. The machine is easy to use with a very well written manual and comes with a user friendly software. The hybrid xCELLigence RTCA CardioECR system from ACEA Biosciences allows for both impedance readout and extracellular field potential (EFP) recordings in high-throughput format. I thoroughly love this machine we have used it twice in two of my first author publications. Latest: PromoCell GmbH receives EXCiPACT certification as manufacturer of pharmaceutical... Latest: Pesticide residue analysis in cannabis: Method fundamentals and confirmation strategies. Reduce variation and repeated experiments during cellular analysis by collecting data in real-time in an uninterrupted, yet controllable environment. The only downside of this instrument is the cost of the plates used. Labelling can in some cases either block the active site of target proteins or lead to significant changes in conformation of the protein that affects its function. Will be publishing data soon. Pretty easy to use, love that you can get data while you're out of the lab. Good for short term response as well as long-term behaviour. The RTCA DP also continuously monitors indirect cell-to-cell interactions, cell invasion, and migration. Verry helpful for non invasive meassurement of proliferation, invation and migration assays. This system works really well also with cells that do not form monolayers, making it much more than just an ECIS system. The S16 (single 16-well plate) model is the smallest within the xCELLigence instrument line. xCELLigence RTCA DP is very easy to use and easy to clean. Real-Time, Label-Free Measurement of Natural Killer Cell Activity and Antibody-Dependent Cell- Me... Real-Time and Label-Free Monitoring of Virus-Mediated Cytopathogenicity, ACEA Biosciences Launches xCELLigence RTCA Software Pro to Further Enhance Immuno-Oncology Experimental Design and Analysis, ACEA Biosciences to be Acquired by Agilent Technologies, Novel xCELLigence Study of Acinetobacter baumannii Biofilms Receives Award, Scientists Studying E. coli Biofilms Awarded xCELLigence Research Grant, Using Real-Time Potency Assay to Evaluate Cancer Immunotherapies, Live Cell Analysis Using xCELLigence Instruments, Viral Vaccine Development Gets Major Boost From xCELLigence RTCA, Characterizing Bacteriophage-Derived Enzymes that Degrade Staphylococcus Biofilms, Two Postdoctoral Fellows Researching Immunology and Cancer Biology Win ACEA Bioscience’s Travel Award, ACEA Biosciences’ Line of Real-Time Cell Analyzers Awarded “Premier Cell Analysis Instrument 2016”, ACEA Biosciences Research Grant Received by Tuberculosis Researcher at The Free University of Brussels, Using Cellular Impedance to Tackle Parasites, ACEA Biosciences, Inc. ", "Easy to use, reliable and flexible to testing. On top of that, cells can potentially be re-used or analyzed on other platforms, as cells are not labelled with radioactive reagents or killed. The supplier does not provide quotations for this particular product through SelectScience. You can search for similar products in our product directory. Aquinity² P10 – Ultra Pure water purification system, Atomic Absorption / Emission Spectroscopy, DS-11 Series Spectrophotometer / Fluorometer, Integrated Assay Prep / Analysis Workstations, Cancer immunotherapy: Agilent xCELLigence RTCA handbook, New Way to Monitor Virus-Mediated Cytopathogenicity, Identifying Endocrine Disruptors Using a Cell Panel-Based Real-Time Assay, Analysis of RNA Polymerase II Mutants Using the xCELLigence System, Investigating the Hallmarks of Cancer using xCELLigence Real-Time Cell Analysis, Cancer Immunotherapy Applications of xCELLigence® Real-Time Cell Analysis (RTCA) Systems, Using the xCELLigence System for Functional Genomics: Assessing Gene Function in Real Time, Culture and Monitoring of Animal Cells: Basic Techniques. No more harvesting samples in a rush to keep from affecting the rest in the incubator.Watch the results appear on your iPad in real-time. Sometimes I find it difficult to interpret the results. Jun 2015 – Jan 2017 1 year 8 months. The data is clean and easily interpreted. Cellartis cardiomyocytes used in combination with this technique form an excellent platform to accurately predict cardiotoxic responses and to screen compound efficacy. Cells were seeded at 3,000 cells/well in 200 μl of complete media (above) in E-plates (ACEA Biosciences, San Diego, California) and grown overnight while monitored with an xCELLigence SP system (ACEA Biosciences) which monitors cellular events in real time by measuring electrical impedance across inter-digitated gold micro-electrodes … BioSpherix's Cytocentric SubChamber Culture System and ACEA Biosciences xCELLigence ® /iCELLigence ™ Real-Time Cellular Analysis Systems BRING IN VIVO TO YOUR IN VITRO ASSAY. The instrument is placed in a standard CO 2 cell culture incubator and interfaces via a … Perform real-time, label-free cellular assays to: Generate more physiologically relevant data. Measure short-term and long-term cellular e˜ects. The company has just introduced the xCELLigence RTCA S16, which is the ideal entry level model for exploratory studies in cell biology. Novel xCELLigence Study of Acinetobacter baumannii Biofilms Receives Award 7/17/2018 NEW YORK (GenomeWeb) – Agilent Technologies announced today that it has agreed to acquire ACEA Biosciences for $250 million in cash. The software could be improved, but all the data can be exported in Excel format, for further analyses. Great value for money, and easy to use. ACEA Biosciences is a biotechnology company founded in 2002 and headquartered in San Diego, California. The results I obtained from my experiments on the RTCA DP system were interesting and as expected. Although the E-plate is expensive, you can get solid results, so value for money is not bad. Privately held ACEA, which is based in San Diego, has most recently developed a line of xCELLigence impedance-based, label-free, real-time cell analysis instruments that are used in immuno-oncology, pre-clinical drug … The IC50 function was also useful in surmising overall activity of novel anticancer drugs. Reduce the time to clinic. It gives us a better understanding of how the cells are behaving and greater insight into our research. ACEA Biosciences launch xCELLigence RTCA software Pro for immunotherapy applications Dec 28, 2018. Most of our results are reproducable. On September 1, 2020, ACEA Biosciences officially joins the Agilent Technologies family. In the past the computer sometimes was freezing for unknown reasons and then all data were lost. The xCELLigence DP is easy to use, provides the flexibility of monitoring cells in real-time and allows the assessment of multiple time points and dual functions in a single experiment, without the need to repeat tedious, conventional endpoint assays numerous times. Furthermore, RTCA detected not only drug-induced changes in cell number but also the kinetics of drug action. The xCELLigence system has been a huge asset to our lab. The small reagent volumes required supports the economical use of limited reagents. Expect from the problem with the USB-port, we would recommend to everybody interested in cell behaviour to buy this xCELLigence device. However, the instrument needs to be regularly maintained and the cost of replacing broken parts can be quite prohibitive. RTCA software was used to determine CI values through the measured impedance recordings. Lable-free and real-time analysis of cell growth and behavior made simple with xCELLIgence RTCA DP. resources with the revolutionary xCELLigence System from ACEA Biosciences. It is currently being used worldwide in industrial and academic labs in applications that include, but are not limited to, the following: Manufacturer Agilent Technologies  |  Available Worldwide. Overall the system was very user friendly and graphs generated were intuitive, informative and reproducible. It is great to be able to monitor our cells and gather data in real time instead of end point assays. Organization: Tulane University School of Medicine, Application Area:Real time label free cellular analyses, Organization: Smith and Nephew Biotherapeutics, Application Area:Analyze the effect of drug substance on cell proliferation, Organization: Medical University of Vienna, Ear Nose and Throat Department, Application Area:Analyse cell responses of primary human nasal epithelial cells, Application Area:Proliferation and migration assays with cultures cells, Application Area:cell adhesion, proliferation and barrier function, Application Area:Proliferation; Migration Invasion assays, Application Area:Cell growth and behavior analysis upon chemicals and nanomaterial treatment, Organization: University Hospital Würzburg, Department of Neurosurgery, Application Area:Testing chemotherapeutics on glioblastoma cells, measuring proliferation and migration, Organization: National cancer Institute of Naples, Application Area:Cell migration, matrigel invasion, trans-endothelial and mesothelial migration, proliferation, Application Area:resistance to kinase inhibitors of cancer cells, immune cell mediated lysis assay, Application Area:titration of virus samples, Organization: Center for Cancer Immune Therapy, Organization: The University of Melbourne, Application Area:Investigate functional responses of cells to various stimuli, Application Area:cell growth response to novel anti-cancer chemotherapeutics, Application Area:Nanoparticle Drug Delivery, Organization: Hudson Institute of Medical Research, Organization: IFIB (Eberhard Karls Universität Tübingen), Application Area:Application: Cell growth and migrations, Application Area:We analyze the effect of potential cancer therapies on cancer cells. ACEA Biosciences Launches xCELLigence RTCA Software Pro to Further Enhance Immuno-Oncology Experimental Design and Analysis 17 Oct 2018. Really easy to use and export experiments. Espacially that you can't safe the data analysis part and also that you always have to click newly what you want to see on the screen, when you open a new file. ACEA Biosciences, Inc. has been a pioneer in the development and commercialization of high-performance microelectronic systems for cell-based assays. ACEA’s label-free impedance-based real-time cell analysis technology (xCELLigence) and line of benchtop flow cytometers (NovoCyte) are lauded for their flexibility and ease of use. What distinguishes the DP (dual purpose) model from our other xCELLigence instruments is its ability to additionally make kinetic measurements of cell invasion and migration (CIM) using an electronically integrated Boyden chamber (CIM-Plate® 16). 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